Here is the spreadsheet on primer bias. I looked the restriction enzymes up in a catalog.
| AFLP Primer | A+T | G+C | |||||||||||||||
| EcoR1/Mse1 | G | A | A | T | T | C | C | T | T | A | A | A | C | 9 | 4 | ||
| EcoR1AG/Mse1G | G | A | A | T | T | C | A | G | T | T | A | A | G | 9 | 4 | ||
| Pst1C/Mse1AC | C | T | G | C | A | G | C | T | T | A | A | A | C | 7 | 6 | ||
| Pst1C/Mse1TG | C | T | G | C | A | G | C | T | T | A | A | T | G | 7 | 6 | ||
| 32 | 20 | ||||||||||||||||
| Selective Bases in Bold | |||||||||||||||||
| GC/AT = | 0.63 | ||||||||||||||||
| RAPD Primers | A+T | G+C | |||||||||||||||
| OPA-01 | C | A | G | G | C | C | C | T | T | C | 3 | 7 | |||||
| OPA-11 | C | A | A | T | C | G | C | C | G | T | 4 | 6 | |||||
| OPA-13 | C | A | G | C | A | C | C | C | A | C | 3 | 7 | |||||
| OPA-14 | T | C | T | G | T | G | C | T | G | G | 4 | 6 | |||||
| OPA-15 | T | T | C | C | G | A | A | C | C | C | 4 | 6 | |||||
| OPA-16 | A | G | C | C | A | G | C | G | A | A | 4 | 6 | |||||
| OPA-17 | G | A | C | C | G | C | T | T | G | T | 4 | 6 | |||||
| OPA-18 | A | G | G | T | G | A | C | C | G | T | 4 | 6 | |||||
| OPA-19 | C | A | A | A | C | G | T | C | G | G | 4 | 6 | |||||
| OPA-20 | G | T | T | G | C | G | A | T | C | C | 4 | 6 | |||||
| 38 | 62 | ||||||||||||||||
| GC/AT = | 1.63 |
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Last Updated May 12, 1999